Generation of human induced pluripotent stem cell line (BCRTi007-A) from urinary cells of a patient with autosomal dominant polycystic kidney disease

Lilas Batool; Constanze Raab; Christien Madlen Beez; Andreas Kurtz; Maik Gollasch; Bella Rossbach

doi:10.1016/j.scr.2023.103071

Generation of human induced pluripotent stem cell line (BCRTi007-A) from urinary cells of a patient with autosomal dominant polycystic kidney disease

Stem Cell Res. 2023 Jun:69:103071. doi: 10.1016/j.scr.2023.103071. Epub 2023 Mar 20.

Authors

Lilas Batool¹, Constanze Raab¹, Christien Madlen Beez², Andreas Kurtz³, Maik Gollasch⁴, Bella Rossbach⁵

Affiliations

¹ Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
² Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Deutsches Herzzentrum der Charité (DHZC), Department of Cardiothoracic and Vascular Surgery, Augustenburger Platz 1, 13353 Berlin, Germany.
³ Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Germany, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany. Electronic address: Andreas.Kurtz@bih-charite.de.
⁴ Klinik und Poliklinik für Innere Medizin D - Geriatrie, Universitätsmedizin Greifswald, Ferdinand-Sauerbruch-Straße, 17489 Greifswald, Germany.
⁵ Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Germany, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany. Electronic address: bella.rossbach@ibmt.fraunhofer.de.

PMID: 36947994
DOI: 10.1016/j.scr.2023.103071

Abstract

Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder of adults, characterized by uncontrolled cysts formation that causes a gradual impairment of kidney function. We generated a human induced pluripotent stem cell (hiPSC) line from the urinary cells of a patient diagnosed with ADPKD using a non-integrating Epi5™ Episomal iPSC reprogramming strategy. Characterization of the cell line was performed regarding their undifferentiated status, differentiation potential, and quality control for karyotypic integrity, identity, and clearance of reprogramming vectors. The newly derived hiPSC line, namely BCRTi007-A, can be used in vitro for disease modeling of ADPKD as well as testing for novel therapeutic approaches.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Cell Line
Humans
Induced Pluripotent Stem Cells* / metabolism
Mutation
Polycystic Kidney, Autosomal Dominant* / genetics
Polycystic Kidney, Autosomal Dominant* / metabolism