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J Chromatogr. 1987 Aug 28;404(1):41-52.

Computer analysis of automated Edman degradation and amino acid analysis data.

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Department of Developmental Biology, Genentech, Inc., South San Francisco, CA 94080.


Computer programs are described that allow facile analysis of data from a protein sequencer and amino acid analyzer. The sequencer program provides automated sequence interpretation while requiring minimal user interaction. The program serves as a powerful aid in deciphering mixture sequences and allows routine monitoring of sequencer performance. The computer program for amino acid analysis data provides the following calculations: mole percent, protein concentration and residues per mole with comparison between theoretical and calculated values. A plot of molecular weight versus deviation from integer values is calculated providing a measure of peptide or protein purity.

[Indexed for MEDLINE]

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