Fluorescence imaging of live cells allows for the observation of dynamic processes inside cells in real time. Here we describe a strategy to image clathrin-coated vesicle dynamics in a single focal plane at the trans-Golgi network of the yeast Saccharomyces cerevisiae. This method can be readily adapted for live cell imaging of a diverse set of dynamic processes within cells.
Keywords: Adaptor; CCV; Clathrin; Dynamic; Golgi; Intracellular; Live cell fluorescence; Spinning disc confocal microscopy; TGN; Vesicle; Yeast.
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