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J Submicrosc Cytol. 1987 Jul;19(3):483-7.

The influence of age of culture on the morphology of T47D cells.


The cell line T47D is extensively used as model in studying the role of hormones in human breast cancer. On the other hand the study of their morphology is largely incidental and does not take into account the age of culture. We studied therefore the morphology of T47D cell line with the electron microscope from the third day of culture to the seventh without adding new medium and serum to find out if the age of culture affects their characteristics. Our results suggest that there is a constant change of the cell morphology and architecture. Thus some cell organelles as the nucleus, tonofilaments, endoplasmic reticulum, mitochondria and intercellular secretory ductules are affected by the age of culture. Also we observed signs of degeneration, necrobiosis and even necrosis on the seventh day of culture. These observations suggest that the age of culture of these cells should be taken into account in experiment planning.

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