Shipi Shugan Decoction Protected against Sequela of Pelvic Inflammatory Disease via Inhibiting SIRT1/NLRP3 Signaling Pathway in Pelvic Inflammatory Disease Rats

Yan Wang; Yefang Huang; Ling Shi; Li Huang; Yi Wen; Yihong Cao; Zi Yang; Qian Liu; Xiaolan Yin; Xiaoli Ji

doi:10.1155/2022/6382205

Shipi Shugan Decoction Protected against Sequela of Pelvic Inflammatory Disease via Inhibiting SIRT1/NLRP3 Signaling Pathway in Pelvic Inflammatory Disease Rats

Evid Based Complement Alternat Med. 2022 Sep 5:2022:6382205. doi: 10.1155/2022/6382205. eCollection 2022.

Authors

Yan Wang¹, Yefang Huang¹, Ling Shi¹, Li Huang¹, Yi Wen¹, Yihong Cao¹, Zi Yang¹, Qian Liu¹, Xiaolan Yin¹, Xiaoli Ji¹

Affiliation

¹ Department of Gynecology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China.

Abstract

Sequela of pelvic inflammatory disease (SPID) is a common and frequently occurring disease clinically. Traditional Chinese medicine (TCM) provided unique advantages in the treatment of SPID. In this study, we aimed to investigate the protective mechanism of Shipi Shugan Decoction (SSD), a Chinese herbal formula, on SPID using a SPID rat model. Mixed bacterial infection and mechanical injury were used for modeling. The chemical composition of SSD was analyzed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA) and western blot techniques. We found that SSD dose-dependently inhibited the content of IL-18, IL-1β, TNF-α, and IL-6 in serum samples of SPID rats. The results from the hematoxylin and eosin (H&E) stain showed that SSD improved pathological injury of the uterus and fallopian tubes induced by a pathogen. In addition, SSD dose-dependently inhibited mitochondrial dysfunction and oxidative stress of SPID rats. The expression of SIRT1 was promoted, and NLRP3 inflammasome was deactivated by SSD gavage compared with the SPID group. Specifically, SIRT1 inhibitor EX-527 cotreatment significantly reversed the improvement effect of SSD on pelvic inflammatory disease in rats. Taken together, the results of this study suggest that Shipi Shugan Decoction may be an effective TCM for the treatment of SPID.