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Ecotoxicol Environ Saf. 1987 Apr;13(2):202-7.

The effects of thiosemicarbazide on development in the wood frog, Rana sylvatica. II. Critical exposure length and age sensitivity.


Wood frog (Rana sylvatica) tadpoles were exposed to 50 mg thiosemicarbazide (TSC)/liter water for varying lengths of time and at different developmental stages. Short exposure periods (3 and 6 hr) resulted in no visible deformities. Exposure periods of 12 or more hr caused slight to severe abnormalities, with a direct relationship between length of exposure and degree of deformity. Tadpoles exposed to TSC from posthatching Days 24-30 were more seriously affected than were tadpoles exposed at an older age.

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