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Nature. 1978 Sep 14;275(5676):104-9.

Transformation of yeast by a replicating hybrid plasmid.

Abstract

Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from E. coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.

PMID:
357984
DOI:
10.1038/275104a0
[Indexed for MEDLINE]

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