Dynamically regulating metabolic fluxes with synthetic metabolons

Youjun Zhang; Alisdair R Fernie

doi:10.1016/j.tibtech.2022.06.005

Dynamically regulating metabolic fluxes with synthetic metabolons

Trends Biotechnol. 2022 Sep;40(9):1019-1020. doi: 10.1016/j.tibtech.2022.06.005. Epub 2022 Jun 23.

Authors

Youjun Zhang¹, Alisdair R Fernie²

Affiliations

¹ Center for Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany. Electronic address: YOZhang@mpimp-golm.mpg.de.
² Center for Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany. Electronic address: fernie@mpimp-golm.mpg.de.

PMID: 35753889
DOI: 10.1016/j.tibtech.2022.06.005

Abstract

Enzyme-enzyme assemblies commonly occur naturally, yet the factors that lead to their transient nature are not fully understood. Mitkas et al. have shown how clustered regularly interspaced short palindromic repeats (CRISPR) enzymes and RNA scaffolds allow synthetic enzyme complexes to be formed and disassembled as needed, providing powerful new tools for metabolic engineering.

Keywords: CRISPR-Cas6; auxin; malate; pathway flux; substrate channeling; synthetic biology.

Publication types

Research Support, Non-U.S. Gov't
Comment

MeSH terms

CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats*
Metabolic Engineering
RNA* / metabolism

Substances

RNA