Long non-coding RNA HOTAIR regulates cytoskeleton remodeling and lipid storage capacity during adipogenesis

Evdokiia Potolitsyna; Sarah Hazell Pickering; Thomas Germier; Philippe Collas; Nolwenn Briand

doi:10.1038/s41598-022-14296-6

Long non-coding RNA HOTAIR regulates cytoskeleton remodeling and lipid storage capacity during adipogenesis

Sci Rep. 2022 Jun 16;12(1):10157. doi: 10.1038/s41598-022-14296-6.

Authors

Evdokiia Potolitsyna¹, Sarah Hazell Pickering^{1

2}, Thomas Germier¹, Philippe Collas^{3

4}, Nolwenn Briand^{5

6}

Affiliations

¹ Department of Molecular Medicine, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Blindern, PO Box 1112, 0317, Oslo, Norway.
² Department of Immunology and Transfusion Medicine, Oslo University Hospital, 0424, Oslo, Norway.
³ Department of Molecular Medicine, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Blindern, PO Box 1112, 0317, Oslo, Norway. philippe.collas@medisin.uio.no.
⁴ Department of Immunology and Transfusion Medicine, Oslo University Hospital, 0424, Oslo, Norway. philippe.collas@medisin.uio.no.
⁵ Department of Molecular Medicine, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Blindern, PO Box 1112, 0317, Oslo, Norway. nolwenn.briand@medisin.uio.no.
⁶ Department of Immunology and Transfusion Medicine, Oslo University Hospital, 0424, Oslo, Norway. nolwenn.briand@medisin.uio.no.

Abstract

The long non-coding RNA HOTAIR is the most differentially expressed gene between upper- and lower-body adipose tissue, yet its functional significance in adipogenesis is unclear. We report that HOTAIR expression is transiently induced during early adipogenic differentiation of gluteofemoral adipose progenitors and repressed in mature adipocytes. Upon adipogenic commitment, HOTAIR regulates protein synthesis pathways and cytoskeleton remodeling with a later impact on mature adipocyte lipid storage capacity. Our results support novel and important functions of HOTAIR in the physiological context of adipogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / metabolism
Adipogenesis* / genetics
Cell Differentiation / genetics
Cell Differentiation / physiology
Cytoskeleton / genetics
Lipids
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism

Substances

Lipids
RNA, Long Noncoding