Cardiovascular and Gas Exchange Effects of Individualized Positive End-Expiratory Pressures in Cats Anesthetized With Isoflurane

Objectives: To compare the effects of four levels of end-expiratory pressure [zero (ZEEP) and three levels of positive end-expiratory pressure (PEEP)] on the cardiovascular system and gas exchange of cats anesthetized with isoflurane and mechanically ventilated for 3 h with a tidal volume of 10 ml/kg.

Study design: Prospective, randomized, controlled trial.

Animals: Six healthy male neutered purpose-bred cats.

Methods: Anesthesia was induced with isoflurane and maintained at 1.3 minimum alveolar concentration. PEEP of maximal respiratory compliance (PEEP_maxCrs) was identified in a decremental PEEP titration, and cats were randomly ventilated for 3 h with one of the following end-expiratory pressures: ZEEP, PEEP_maxCrs minus 2 cmH₂O (PEEP_maxCrs-2), PEEP_maxCrs, and PEEP_maxCrs plus 2 cmH₂O (PEEP_maxCrs+2). Cardiovascular and gas exchange variables were recorded at 5, 30, 60, 120, and 180 min (T5 to T180, respectively) of ventilation and compared between and within ventilation treatments with mixed-model ANOVA followed by Dunnet's and Tukey's tests (normal distribution) or Friedman test followed by the Dunn's test (non-normal distribution). Significance to reject the null hypothesis was considered p < 0.05.

Results: Mean arterial pressure (MAP-mmHg) was lower in PEEP_maxCrs+2 [63 (49-69); median (range)] when compared to ZEEP [71 (67-113)] at T5 and stroke index (ml/beat/kg) was lower in PEEP_maxCrs+2 (0.70 ± 0.20; mean ± SD) than in ZEEP (0.90 ± 0.20) at T60. Cardiac index, oxygen delivery index (DO₂I), systemic vascular resistance index, and shunt fraction were not significantly different between treatments. The ratio between arterial partial pressure and inspired concentration of oxygen (PaO₂/FIO₂) was lower in ZEEP than in the PEEP treatments at various time points. At T180, DO₂I was higher when compared to T5 in PEEP_maxCrs. Dopamine was required to maintain MAP higher than 60 mmHg in one cat during PEEP_maxCrs and in three cats during PEEP_maxCrs+2.

Conclusion: In cats anesthetized with isoflurane and mechanically ventilated for 3 h, all levels of PEEP mildly improved gas exchange with no significant difference in DO₂I when compared to ZEEP. The PEEP levels higher than PEEP_maxCrs-2 caused more cardiovascular depression, and dopamine was an effective treatment. A temporal increase in DO₂I was observed in the cats ventilated with PEEP_maxCrs. The effects of these levels of PEEP on respiratory mechanics, ventilation-induced lung injury, as well as in obese and critically ill cats deserve future investigation for a better understanding of the clinical use of PEEP in this species.