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J Immunoassay. 1987;8(1):29-41.

The use of 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay.

Abstract

The assay conditions for the use of 3,3',5,5' tetramethylbenzidine (TMB) in microplate enzyme-linked immunosorbent assay are described. TMB is a safe (non-mutagenic) chromogen that is more sensitive than OPD as a substrate for horseradish peroxidase. We describe the optimum storage and assay conditions for this chromogen.

PMID:
3558827
DOI:
10.1080/01971528708063053
[Indexed for MEDLINE]

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