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J Neurosci Methods. 1986 May;16(3):227-38.

Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems.

Abstract

A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The characteristic morphology of neurons is maintained, together with the diversity of active channels evident in the intact nervous system.

PMID:
3523050
[Indexed for MEDLINE]

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