Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

Guilherme O Barbosa; Thomas B Kornberg

doi:10.1016/j.xpro.2022.101138

Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

STAR Protoc. 2022 Jan 29;3(1):101138. doi: 10.1016/j.xpro.2022.101138. eCollection 2022 Mar 18.

Authors

Guilherme O Barbosa¹, Thomas B Kornberg¹

Affiliation

¹ Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA.

Abstract

This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-Studios pipeline. It can also be used for studies of cell division, organelle localization, and protein trafficking as well as other cellular materials that can be fluorescently tagged and imaged with minimal phototoxicity.

Keywords: Cell Biology; Developmental biology; Microscopy; Model Organisms.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Drosophila / metabolism
Drosophila Proteins* / metabolism
Drosophila melanogaster*
Imaginal Discs
Signal Transduction
Time-Lapse Imaging

Substances

Drosophila Proteins

Grants and funding

R35 GM122548/GM/NIGMS NIH HHS/United States