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Mutat Res. 1986 Jan;165(1):39-44.

Postreplicational formation and repair of DNA double-strand breaks in UV-irradiated Escherichia coli uvrB cells.

Abstract

The number of DNA double-strand breaks formed in UV-irradiated uvrB recF recB cells correlates with the number of unrepaired DNA daughter-strand gaps, and is dependent on DNA synthesis after UV-irradiation. These results are consistent with the model that the DNA double-strand breaks that are produced in UV-irradiated excision-deficient cells occur as the result of breaks in the parental DNA opposite unrepaired DNA daughter-strand gaps. By employing a temperature-sensitive recA200 mutation, we have devised an improved assay for studying the formation and repair of these DNA double-strand breaks. Possible mechanisms for the postreplication repair of DNA double-strand breaks are discussed.

PMID:
3510373
DOI:
10.1016/0167-8817(86)90007-6
[Indexed for MEDLINE]

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