Role of miR-100-5p and CDC25A in breast carcinoma cells

Xiaoping Li; Yanli Ren; Donghong Liu; Xi Yu; Keda Chen

doi:10.7717/peerj.12263

Role of miR-100-5p and CDC25A in breast carcinoma cells

PeerJ. 2022 Jan 3:9:e12263. doi: 10.7717/peerj.12263. eCollection 2022.

Authors

Xiaoping Li^{1

2}, Yanli Ren³, Donghong Liu⁴, Xi Yu¹, Keda Chen²

Affiliations

¹ Faculty of Medicine, Macau University of Science and Technology, Macau, China.
² Shulan International Medical College, Zhejiang Shuren University, Hangzhou, Zhejiang province, China.
³ State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang province, China.
⁴ Department of Laboratory Medicine, Hangyan hospital of Wenzhou Medical University, Taizhou First People's Hospital, Taizhou, Zhejiang, China.

Abstract

Objective: To inquiry about mechanism of miR-100-5p/CDC25A axis in breast carcinoma (BC), thus offering a new direction for BC targeted treatment.

Methods: qRT-PCR was employed to explore miR-100-5p and CDC25A mRNA levels. Western blot was employed for detecting protein expression of CDC25A. Targeting relationship of miR-100-5p and CDC25A was verified by dual-luciferase assay. In vitro experiments were used for assessment of cell functions.

Results: In BC tissue and cells, miR-100-5p was significantly lowly expressed (P < 0.05) while CDC25A was highly expressed. Besides, miR-100-5p downregulated CDC25A level. miR-100-5p had a marked influence on the prognosis of patients. The forced miR-100-5p expression hindered BC cell proliferation, migration and invasion, and facilitated cell apoptosis. Upregulated miR-100-5p weakened promotion of CDC25A on BC cell growth.

Conclusion: Together, these findings unveiled that CDC25A may be a key target of miR-100-5p that mediated progression of BC cells. Hence, miR-100-5p overexpression or CDC25A suppression may contribute to BC diagnosis.

Keywords: Breast carcinoma; CDC25A; MiR-100-5p; Migration and invasion; Proliferation.

Grants and funding

This study was supported by the Zhejiang provincial basic public welfare research program (LGF21H200007) and the Public Welfare Technology Applied Research Program of Zhejiang Province (2017C37020). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.