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J Immunol Methods. 1986 Jun 10;90(1):105-10.

A rapid ELISA for measurement of antibodies to nucleic acid antigens using UV-treated polystyrene microplates.

Abstract

Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly(L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematous patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.

PMID:
3486920
[Indexed for MEDLINE]

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