Positive control of D-serine deaminase synthesis in vitro

Proc Natl Acad Sci U S A. 1978 Apr;75(4):1695-9. doi: 10.1073/pnas.75.4.1695.

Abstract

Efficient constitutive synthesis of D-serine deaminase [D-serine hydro-lyase (deaminating); EC 4.2.1.14] is obtained in vitro by using a slightly modified Zubay system programmed with dsdO6 dsdA+DNA. Synthesis from a dsdO+ dsdA+ template requires active dsdC gene product and 3':5'-cyclic AMP. In vitro synthesis of dsdC product is obtained with a dsdC+ dsdO+ dsdA+ or a dsdCc dsdO+ dsdA+ template; this synthesis is thermosensitive and can be uncoupled from D-serine deaminase synthesis by temperature shift.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell-Free System
  • Coliphages / genetics
  • Enzyme Induction
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Genes*
  • Genes, Regulator*
  • L-Serine Dehydratase / biosynthesis
  • L-Serine Dehydratase / genetics*
  • Operon*
  • Serine / pharmacology
  • Templates, Genetic

Substances

  • Serine
  • L-Serine Dehydratase