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Gene. 1987;61(1):1-11.

Design and construction of a versatile system for the expression of foreign genes in plants.

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Department of Plant Pathology, University of Kentucky, Lexington 40546-0091.


We have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. These vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35S promoter or the pea rbcS-E9 promoter, a multiple cloning site derived from M13um20, and the rbcS-E9 polyadenylation site. These cassettes have been incorporated into pBR322-based or RK2-based replicons to facilitate direct DNA uptake or Agrobacterium tumefaciens-mediated gene transfer. Their application for the expression of a bacterial gene is described.

[Indexed for MEDLINE]

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