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Gene. 1987;60(1):65-74.

Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter.

Author information

1
Department of Medicine, University of Ottawa, Ont., Canada.

Abstract

We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp 1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse pgk-1 gene is homologous to the human pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene.

PMID:
3440520
DOI:
10.1016/0378-1119(87)90214-9
[Indexed for MEDLINE]

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