Circular RNA TADA2A promotes proliferation and migration via modulating of miR‑638/KIAA0101 signal in non‑small cell lung cancer

Ye Zhang; Hongmin Yao; Ying Li; Lu Yang; Liang Zhang; Jinxin Chen; Yong Wang; Xia Li

doi:10.3892/or.2021.8152

Circular RNA TADA2A promotes proliferation and migration via modulating of miR‑638/KIAA0101 signal in non‑small cell lung cancer

Oncol Rep. 2021 Sep;46(3):201. doi: 10.3892/or.2021.8152. Epub 2021 Jul 23.

Authors

Ye Zhang¹, Hongmin Yao¹, Ying Li¹, Lu Yang², Liang Zhang³, Jinxin Chen⁴, Yong Wang⁵, Xia Li¹

Affiliations

¹ Department of Radiation Oncology, Cancer Hospital of China Medical University/Liaoning Cancer Hospital and Institute, Dadong, Shenyang, Liaoning 110042, P.R. China.
² First Department of Gastroenterology, Cancer Hospital of China Medical University/Liaoning Cancer Hospital and Institute, Dadong, Shenyang, Liaoning 110042, P.R. China.
³ Department of Breast Internal Medicine, Cancer Hospital of China Medical University/Liaoning Cancer Hospital and Institute, Dadong, Shenyang, Liaoning 110042, P.R. China.
⁴ Department of Gynecological Oncology, Cancer Hospital of China Medical University/Liaoning Cancer Hospital and Institute, Dadong, Shenyang, Liaoning 110042, P.R. China.
⁵ Central Laboratory, Central Hospital Affiliated to Shenyang Medical College, Dadong, Shenyang, Liaoning 110024, P.R. China.

Abstract

Accumulating evidence indicates that circular (circ)RNAs exhibit complex functions in diverse malignant tumors, including non‑small cell lung cancer (NSCLC). The role of the circRNA transcription adaptor 2A (circTADA2A) in NSCLC remains unclear. The expression, function and mechanism of circTADA2A in NSCLC development were investigated in the present study. The results revealed that circTADA2A was upregulated in NSCLC, and that knockdown of circTADA2A inhibited cell proliferation and migration in the NSCLC cell lines A549 and H1299. Functional assays demonstrated that circTADA2A promoted proliferation and migration via interacting with microRNA (miR)‑638. Bioinformatics and reverse transcription‑quantitative PCR assay confirmed that miR‑638 was expressed at low levels in NSCLC. In addition, it was found that miR‑638 served a tumor‑suppressive role and suppressed proliferation and migration via PCNA clamp associated factor (KIAA0101) inhibition in A549 and H1299 cells. Lastly, it was verified that circTADA2A promoted cell proliferation and migration, at least partially, via miR‑638/KIAA0101 signaling in A549 and H1299 cells. In summary, the present study showed that circTADA2A promoted NSCLC cell proliferation and migration via modulating miR‑638/KIAA0101 signaling.

Keywords: PCNA clamp associated factor; circular RNA transcription adaptor 2A; microRNA‑638; migration; non‑small cell lung cancer; proliferation.

MeSH terms

A549 Cells
Aged
Aged, 80 and over
Area Under Curve
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / metabolism*
Cell Movement
Cell Proliferation
DEAD-box RNA Helicases / genetics
DEAD-box RNA Helicases / metabolism*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Dactinomycin / metabolism
Eukaryotic Initiation Factor-4A / genetics
Eukaryotic Initiation Factor-4A / metabolism*
Exoribonucleases / metabolism
Female
Gene Expression Regulation, Neoplastic*
Humans
Lung Neoplasms / genetics*
Lung Neoplasms / metabolism*
Male
MicroRNAs / genetics
MicroRNAs / metabolism*
Middle Aged
Prognosis
Proliferating Cell Nuclear Antigen / metabolism
RNA, Circular*
Transcription Factors / genetics
Transcription Factors / metabolism*
Up-Regulation

Substances

DNA-Binding Proteins
MIRN638 microRNA, human
MicroRNAs
PCNA protein, human
Proliferating Cell Nuclear Antigen
RNA, Circular
TADA2A protein, human
Transcription Factors
Dactinomycin
Eukaryotic Initiation Factor-4A
Exoribonucleases
ribonuclease R
EIF4A3 protein, human
DEAD-box RNA Helicases

Grants and funding

The present study was supported by grants from Natural Science Foundation of Liaoning Province (grant no. 20180550318), Youth Talent Support Program of Liaoning Province (grant no. XLYC1907011), Key R&D Program of Liaoning Province (grant no. 2018225014) and Technological Innovation Fund of Shenyang Technology Division (grant nos. RC190008 and 19-112-4-023).