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Biol Chem Hoppe Seyler. 1987 Oct;368(10):1385-90.

The primary structure of the hemoglobins of the adult jaguar (Panthera onco, Carnivora).

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Max-Planck-Institut für Biochemie, Abt. Proteinchemie, Martinsried bei München.


The primary structure of the hemoglobins from Jaguar (Panthera onco) are presented. Electrophoretic separations without and with a dissociating agent revealed the presence of two hemoglobin components, alpha 2 beta I2 and alpha 2 beta II2. The separation of the hemoglobin components was achieved by ion-exchange chromatography. The globin chains were separated by ion-exchange chromatography and also by reversed phase HPLC. The amino-acid sequences of the native chains and peptides were determined by liquid-phase and gas-phase sequencing. N-Acetylserine was detected by FAB-mass spectroscopy as N-terminal group of the beta I chain. The sequences are compared with that of human hemoglobin (Hb A).

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