Anti-calcitonin gene-related peptide antibody attenuates orofacial mechanical and heat hypersensitivities induced by infraorbital nerve injury

Hiroharu Maegawa; Chiaki Yoshikawa; Nayuka Usami; Hiroshi Hanamoto; Chiho Kudo; Hitoshi Niwa

doi:10.1016/j.bbrc.2021.07.001

Anti-calcitonin gene-related peptide antibody attenuates orofacial mechanical and heat hypersensitivities induced by infraorbital nerve injury

Biochem Biophys Res Commun. 2021 Sep 10:569:147-153. doi: 10.1016/j.bbrc.2021.07.001. Epub 2021 Jul 8.

Authors

Hiroharu Maegawa¹, Chiaki Yoshikawa², Nayuka Usami³, Hiroshi Hanamoto⁴, Chiho Kudo⁵, Hitoshi Niwa⁶

Affiliations

¹ Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: maegawa@dent.osaka-u.ac.jp.
² Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: cyoshikawa@dent.osaka-u.ac.jp.
³ Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: nayuka.usami@dent.osaka-u.ac.jp.
⁴ Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: hanamoto@dent.osaka-u.ac.jp.
⁵ Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: ckudo@dent.osaka-u.ac.jp.
⁶ Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Suita, Osaka 565-0871, Japan. Electronic address: niwa@dent.osaka-u.ac.jp.

PMID: 34245979
DOI: 10.1016/j.bbrc.2021.07.001

Abstract

Currently, limited information regarding the role of calcitonin gene-related peptide (CGRP) in neuropathic pain is available. Intracerebroventricular administrations of an anti-CGRP antibody were performed in rats with infraorbital nerve ligation. Anti-CGRP antibody administration attenuated mechanical and heat hypersensitivities induced by nerve ligation and decreased the phosphorylated extracellular signal-regulated kinase expression levels in the trigeminal spinal subnucleus caudalis (Vc) following mechanical or heat stimulation. An increased CGRP immunoreactivity in the Vc appeared after nerve ligation. A decreased CGRP immunoreactivity resulted from anti-CGRP antibody administration. Our findings suggest that anti-CGRP antibody administration attenuates the symptoms of trigeminal neuropathic pain by acting on CGRP in the Vc.

Keywords: Calcitonin gene-related peptide; Chronic constriction injury; Orofacial sensory hypersensitivity; Phosphorylated extracellular signal-regulated kinase; Trigeminal spinal subnucleus caudalis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / administration & dosage
Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / pharmacology*
Calcitonin Gene-Related Peptide / immunology*
Calcitonin Gene-Related Peptide / metabolism
Extracellular Signal-Regulated MAP Kinases / metabolism
Hot Temperature*
Hypersensitivity / etiology
Hypersensitivity / prevention & control*
Immunohistochemistry
Male
Microscopy, Confocal
Neuralgia / etiology
Neuralgia / prevention & control
Phosphorylation
Rats
Rats, Wistar
Stress, Mechanical*
Trigeminal Nerve Injuries / complications*
Trigeminal Nucleus, Spinal / metabolism

Substances

Antibodies, Monoclonal
Extracellular Signal-Regulated MAP Kinases
Calcitonin Gene-Related Peptide