SMARCC2 combined with c‑Myc inhibits the migration and invasion of glioma cells via modulation of the Wnt/β‑catenin signaling pathway

Mol Med Rep. 2021 Aug;24(2):551. doi: 10.3892/mmr.2021.12190. Epub 2021 Jun 3.

Abstract

Glioma is the most common type of central nervous system tumor. SWItch/sucrose non‑fermentable (SWI/SNF) is a tumor suppressor that serves an important role in epithelial‑mesenchymal transition (EMT). The present study aimed to identify key molecules involved in the EMT process. SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2 (SMARCC2) is mutated in and its expression is low in multiple types of cancer. SMARCC2 is the core subunit of the chromatin‑remodeling complex, SWI/SNF. Relative mRNA SMARCC2 expression levels in human glioma tissue were analyzed via reverse transcription‑quantitative PCR, whereas the protein expression levels were determined via immunohistochemistry staining. SMARCC2 expression was knocked down in glioma cells using small interfering RNA (si) and overexpressed by infection with adenovirus vectors carrying SMARCC2 cDNA. Wound healing and Transwell assays were performed to assess cell migration and invasion, respectively. Subsequently, immunofluorescence and western blotting were performed to analyze the expression levels of the oncogene c‑Myc, which is associated with SMARCC2. SMARCC2 combines with C‑MYC to downregulate its expression. Consistent with the results of the bioinformatics analysis, which revealed that the upregulated expression levels of SMARCC2 were associated with a more favorable prognosis in patients with glioma, the mRNA and protein expression levels of SMARCC2 were significantly upregulated in low‑grade glioma tissues compared with high‑grade glioma tissues. The results of the wound healing assay demonstrated that cell migration was significantly increased in the siSMARCC2‑1/3 groups compared with the negative control (NC) group. By contrast, the migratory ability of cells was significantly reduced following transduction with adenovirus overexpressing SMARCC2, which upregulated the expression of SMARCC2, compared with the lentiviral vector‑non‑specific control (LVS‑NC) group. The Transwell assay results further showed that SMARCC2 overexpression significantly inhibited the migratory and invasive abilities of U87MG and LN229 cells compared with the LVS‑NC group. Co‑immunoprecipitation assays were subsequently conducted to validate the binding of SMARCC2 and c‑Myc; the results demonstrated that the expression of c‑Myc was downregulated in adenovirus‑transfected cells compared with LVS‑NC‑transfected cells. The results of the western blotting experiments demonstrated that the expression levels of N‑cadherin, vimentin, snail family transcriptional repressor 1 and β‑catenin were notably downregulated, whereas the expression levels of T‑cadherin were markedly upregulated in cell lines stably overexpressing SMARCC2 compared with the LVS‑NC group. In conclusion, the results of the present study suggested that SMARCC2 may inhibit Wnt/β‑catenin signaling by regulating c‑Myc expression in glioma. SMARCC2 regulates the EMT status of the glioblastoma cell line by mediating the expression of the oncogene C‑MYC to inhibit its migration and invasion ability. Thus, SMARCC2 may function as a tumor suppressor or oncogene by regulating associated oncogenes or tumor suppressor genes.

Keywords: SWI/SNF related; SWItch/sucrose non‑fermentable; actin dependent regulator of chromatin subfamily c member 2; c‑Myc; epithelial‑mesenchymal transition; glioma; matrix associated.

MeSH terms

  • Adult
  • Aged
  • Cadherins / metabolism
  • Cell Line, Tumor
  • Cell Movement / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Epithelial-Mesenchymal Transition / genetics
  • Female
  • Gene Knockdown Techniques
  • Glioma / genetics*
  • Glioma / metabolism
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Invasiveness / genetics
  • Proto-Oncogene Proteins c-myc / genetics*
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Snail Family Transcription Factors / metabolism
  • Survival Analysis
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*
  • Vimentin / metabolism
  • Wnt Signaling Pathway / genetics*
  • Young Adult
  • beta Catenin / metabolism

Substances

  • CTNNB1 protein, human
  • Cadherins
  • DNA-Binding Proteins
  • H-cadherin
  • Proto-Oncogene Proteins c-myc
  • SMARCC2 protein, human
  • SNAI1 protein, human
  • Snail Family Transcription Factors
  • Transcription Factors
  • Vimentin
  • beta Catenin

Grants and funding

The present study was supported by the National Natural Science Foundation of China (grant no. 81802830).