Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification

Nicole Hedley; D Grant Richards; Enzo A Palombo

doi:10.1016/j.mimet.2021.106257

Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification

J Microbiol Methods. 2021 Aug:187:106257. doi: 10.1016/j.mimet.2021.106257. Epub 2021 May 29.

Authors

Nicole Hedley¹, D Grant Richards², Enzo A Palombo³

Affiliations

¹ Department of Chemistry and Biotechnology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia. Electronic address: nhedley@swin.edu.au.
² Gretals Australia, Unit 8/1140 Nepean Highway, Mornington, VIC 3931, Australia.
³ Department of Chemistry and Biotechnology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia.

PMID: 34062212
DOI: 10.1016/j.mimet.2021.106257

Abstract

Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.

Keywords: Gastrointestinal nematodes; Multiplex; Polymerase Chain Reaction; Primers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Primers
DNA, Helminth / isolation & purification*
Feces / parasitology*
Gastrointestinal Tract / parasitology
Multiplex Polymerase Chain Reaction
Nematoda / classification*
Nematoda / genetics
Nematoda / isolation & purification
Nematode Infections / parasitology
Nematode Infections / veterinary*
Ovum
Parasitology / methods*
Polymerase Chain Reaction*
Sheep
Sheep Diseases / parasitology

Substances

DNA Primers
DNA, Helminth