MYOD modified mRNA drives direct on-chip programming of human pluripotent stem cells into skeletal myocytes

Giulia Selmin; Onelia Gagliano; Paolo De Coppi; Elena Serena; Anna Urciuolo; Nicola Elvassore

doi:10.1016/j.bbrc.2021.04.129

MYOD modified mRNA drives direct on-chip programming of human pluripotent stem cells into skeletal myocytes

Biochem Biophys Res Commun. 2021 Jun 30:560:139-145. doi: 10.1016/j.bbrc.2021.04.129. Epub 2021 May 11.

Authors

Giulia Selmin¹, Onelia Gagliano², Paolo De Coppi¹, Elena Serena³, Anna Urciuolo⁴, Nicola Elvassore⁵

Affiliations

¹ Great Ormond Street Institute of Child Health, University College London, WC1N1EH, London, UK.
² Venetian Institute of Molecular Medicine (VIMM), 35129, Padova, Italy; Industrial Engineering Department, University of Padova, 35131, Padova, Italy.
³ Venetian Institute of Molecular Medicine (VIMM), 35129, Padova, Italy.
⁴ Great Ormond Street Institute of Child Health, University College London, WC1N1EH, London, UK; Molecular Medicine Department, University of Padova, Italy.
⁵ Great Ormond Street Institute of Child Health, University College London, WC1N1EH, London, UK; Venetian Institute of Molecular Medicine (VIMM), 35129, Padova, Italy; Industrial Engineering Department, University of Padova, 35131, Padova, Italy. Electronic address: n.elvassore@ucl.ac.uk.

PMID: 33989905
DOI: 10.1016/j.bbrc.2021.04.129

Abstract

Drug screening and disease modelling for skeletal muscle related pathologies would strongly benefit from the integration of myogenic cells derived from human pluripotent stem cells within miniaturized cell culture devices, such as microfluidic platform. Here, we identified the optimal culture conditions that allow direct differentiation of human pluripotent stem cells in myogenic cells within microfluidic devices. Myogenic cells are efficiently derived from both human embryonic (hESC) or induced pluripotent stem cells (hiPSC) in eleven days by combining small molecules and non-integrating modified mRNA (mmRNA) encoding for the master myogenic transcription factor MYOD. Our work opens new perspective for the development of patient-specific platforms in which a one-step myogenic differentiation could be used to generate skeletal muscle on-a-chip.

Keywords: Microfludic; Myoblasts; Pluripotent stem cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics*
Cell Line
Humans
Lab-On-A-Chip Devices
Mesoderm / cytology
Muscle Development
Muscle Fibers, Skeletal / cytology*
MyoD Protein / genetics*
Pluripotent Stem Cells / cytology*
RNA, Messenger
Transfection

Substances

MyoD Protein
RNA, Messenger