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J Chromatogr. 1988 Apr 8;426(1):93-101.

Simultaneous determination of imipramine, desipramine and their deuterium-labelled analogues in biological fluids by capillary gas chromatography-mass spectrometry.

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Department of Physical and Pharmaceutical Chemistry, Tokyo College of Pharmacy, Japan.


A specific, sensitive and accurate method for the determination of imipramine, desipramine and their d4 analogues in biological fluids using d8 analogues as internal standards using capillary gas chromatography-chemical ionization mass spectrometry was developed. Drug concentrations were measured by selected-ion monitoring of the quasi-molecular ions of imipramine and the trifluoro-acetyl derivative of desipramine. The coefficient of variation and relative error at a concentration of about 2 ng/ml in plasma were found less than 10% and 7%, respectively, for both drugs. No isotope effect was observed following the oral administration of an equimolar mixture of imipramine and [2H4]imipramine to a rat.

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