Format

Send to

Choose Destination
Nucleic Acids Res. 1988 May 25;16(10):4637-50.

The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.

Author information

1
Genetics Institute Inc., Cambridge, MA 02140.

Abstract

In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on DNA melting and establish conditions under which oligonucleotides can be used as hybridization probes with predictable and controllable specificity.

PMID:
3380691
PMCID:
PMC336654
DOI:
10.1093/nar/16.10.4637
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center