Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background

Qiuting Dai; Weihao Di; Yi Zhang; Shixin Gu; Jian Zhao; Bei Zhang; Ying Wang

doi:10.1016/j.scr.2021.102314

Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background

Stem Cell Res. 2021 May:53:102314. doi: 10.1016/j.scr.2021.102314. Epub 2021 Mar 26.

Authors

Qiuting Dai¹, Weihao Di¹, Yi Zhang², Shixin Gu², Jian Zhao¹, Bei Zhang³, Ying Wang⁴

Affiliations

¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
² Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China.
³ Department of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
⁴ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China. Electronic address: wangying1@shanghaitech.edu.cn.

PMID: 33799282
DOI: 10.1016/j.scr.2021.102314

Abstract

A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
China
Female
Genetic Background
Humans
Induced Pluripotent Stem Cells*
Kruppel-Like Factor 4
Leukocytes, Mononuclear
Transcription Factors

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Transcription Factors