The results of this investigation indicate that 5 X 10(6) and 5 X 10(8) viable bacillus Calmette-Guerin (BCG) organisms (Mycobacterium bovis strain) can be safely administered in the subconjunctival region of the eye of New Zealand albino rabbits without producing toxic effects locally or within the eye. This study provides a format for testing the applicability of BCG immunotherapy for experimental melanomas.