Format

Send to

Choose Destination
See comment in PubMed Commons below
Gene. 1988;62(1):121-6.

Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker.

Author information

1
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.

Abstract

Recombinant plasmids have been obtained that lead to the accumulation of five- to ten-fold more puromycin-N-acetyl-transferase (PAC) mRNA and two- to three-fold more PAC activity than the already described plasmid pSV2pac [Vara et al., Nucl. Acids Res. 14 (1986) 4117-4124]. When these optimized recombinants were used for stable transformation to puromycin resistance, efficiencies up to 1 x 10(-2) were obtained, indicating that these pac-containing recombinants may be very useful dominant selectable markers for gene transfer in mammalian cells.

PMID:
3371661
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center