Maternal and offspring high-fat diet leads to platelet hyperactivation in male mice offspring

Sci Rep. 2021 Jan 14;11(1):1473. doi: 10.1038/s41598-020-80373-3.

Abstract

Maternal over-nutrition increases the risk of diabetes and cardiovascular events in offspring. While prominent effects on cardiovascular health are observed, the impact on platelet physiology has not been studied. Here, we examined whether maternal high-fat diet (HF) ingestion affects the platelet function in lean and obese offspring. C57BL6/N mice dams were given a HF or control (C) diet for 8 weeks before and during pregnancy. Male and female offspring received C or HF diets for 26 weeks. Experimental groups were: C/C, dam and offspring fed standard laboratory diet; C/HF dam fed standard laboratory diet and offspring fed HF diet; HF/C and HF/HF. Phenotypic and metabolic tests were performed and blood collected for platelet studies. Compared to C/C, offspring HF groups were obese, with fat accumulation, hyperglycaemia and insulin resistance. Female offspring did not present platelet hyperactivity, hence we focused on male offspring. Platelets from HF/HF mice were larger, hyperactive and presented oxidative stress when compared to C/C. Maternal and offspring HF diet results in platelet hyperactivation in male mouse offspring, suggesting a novel 'double-hit' effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiposity / physiology
  • Animals
  • Blood Platelets / physiology
  • Diet, High-Fat / adverse effects*
  • Female
  • Hypertension / physiopathology
  • Insulin Resistance / physiology
  • Lactation
  • Male
  • Maternal Nutritional Physiological Phenomena / physiology*
  • Metabolic Syndrome / metabolism
  • Metabolic Syndrome / physiopathology
  • Mice
  • Mice, Inbred C57BL
  • Obesity / physiopathology
  • Platelet Activation / drug effects
  • Platelet Activation / physiology*
  • Pregnancy
  • Prenatal Exposure Delayed Effects
  • Weaning