Format

Send to

Choose Destination
See comment in PubMed Commons below
J Cereb Blood Flow Metab. 1988 Apr;8(2):282-4.

Quantitative evaluation of vascular permeability in the gerbil brain after transient ischemia using Evans blue fluorescence.

Author information

1
Fifth Department of Internal Medicine, Hyogo College of Medicine, Japan.

Abstract

Mongolian gerbils were used to evaluate brain edema during restoration of flow following bilateral carotid occlusion for 1 h. We have modified the method for fluorometric measurement of Evans blue to monitor vascular protein leakage (vasogenic edema). The extraction of extravasated Evans blue was performed by homogenizing the whole brain in 50% trichloroacetic acid. The supernatant was diluted fourfold with ethanol and the Evans blue fluorescence was measured. The tissue blank was negligible. Evans blue content of the plasma was similarly determined and the ratio of tissue to plasma Evans blue content was calculated. Furthermore, Evans blue fluorescence was used for microscopic investigation. It is suggested that Evans blue fluorescence can be applied for quantification of protein leakage with much more sensitivity and accuracy than the colorimetric absorbance method, as well as for tissue localization of protein leakage.

PMID:
3343300
DOI:
10.1038/jcbfm.1988.59
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Atypon
    Loading ...
    Support Center