Generation of two induced pluripotent stem cell lines SPPHi001-A and SPPHi002-A from a Huntington's disease family of Southwestern China

Bin Li; Fang Ye; Li Chen; Xiangyu Yang; Ji Zhang; Yun Luo; Jie Xu; Yongjie Luo; Su Wang

doi:10.1016/j.scr.2020.102149

Generation of two induced pluripotent stem cell lines SPPHi001-A and SPPHi002-A from a Huntington's disease family of Southwestern China

Stem Cell Res. 2021 Jan 4:50:102149. doi: 10.1016/j.scr.2020.102149. Online ahead of print.

Authors

Bin Li¹, Fang Ye¹, Li Chen², Xiangyu Yang², Ji Zhang², Yun Luo¹, Jie Xu², Yongjie Luo³, Su Wang⁴

Affiliations

¹ Department of Neurology, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China; Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China.
² Department of Neurology, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China; Laboratory for Stem Cell Research, Medical College of University of Electronic Science and Technology of China, Chengdu, China.
³ Department of Neurology, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China; Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China. Electronic address: younglielsc@163.com.
⁴ Department of Neurology, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, China; Laboratory for Stem Cell Research, Medical College of University of Electronic Science and Technology of China, Chengdu, China; Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China. Electronic address: swangneuro@icloud.com.

PMID: 33429129
DOI: 10.1016/j.scr.2020.102149

Abstract

The Huntington's disease (HD) is still a devastating neurological disease without a specific cure. In this report, the peripheral blood mononuclear cells (PBMCs) from two members of a HD family from Southwestern China were reprogrammed into hiPSC lines by an episomal and non-integrating Sendai virus delivery method. The induced HD-hiPSC lines, SPPHi001-A and SPPHi002-A, were expanded and validated for their stem cell-like pluripotency, normal karyotyping, and capability of in vitro differentiation into three germ-layers. These newly generated HD hiPSC lines from Chinese HD patients enable us to further elucidate the mechanism of HD and discover its specific treatment.