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Biochem Biophys Res Commun. 1988 Jan 15;150(1):376-83.

Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties.

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Department of Medicine, University of California Medical Center, San Francisco 94143.


Arachidonate 15-lipoxygenase was purified from human eosinophil-enriched leukocytes after showing that 15-lipoxygenase activity was 100-fold greater in eosinophils than in neutrophils. Partial purification was achieved using ammonium sulfate precipitation, cation-exchange and hydrophobic-interaction chromatography. New evidence is presented suggesting that 15-lipoxygenase has electrostatic and hydrophobic properties distinct from 5-lipoxygenase. In addition, ATP is shown to inhibit, and phosphatidylcholine is shown to stimulate, 15-lipoxygenase, suggesting a regulatory role for these compounds in the lipoxygenation of arachidonic acid.

[Indexed for MEDLINE]

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