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J Gen Microbiol. 1987 Oct;133(10):2719-25.

Identification of the Escherichia coli cysM gene encoding O-acetylserine sulphydrylase B by cloning with mini-Mu-lac containing a plasmid replicon.

Author information

1
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

Abstract

A region located at around 52' on the Escherichia coli chromosome was cloned by use of mini-Mu-lac containing a plasmid replicon and recloned into pBR322. Enzyme assays on transformants carrying the cloned fragments indicated the presence in the latter of the cysA and cysM genes coding for sulphate permease and O-acetylserine sulphydrylase B, respectively.

PMID:
3329675
DOI:
10.1099/00221287-133-10-2719
[Indexed for MEDLINE]

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