Send to

Choose Destination
Mol Gen Genet. 1987 Nov;210(1):101-10.

Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid.

Author information

Centro de Investigaciones Biológicas (C.S.I.C.), Velázquez, Madrid, Spain.


We provide evidence that a mutation which derepresses an autoregulated system that is located in the vicinity of the basic replicon of R1, stabilizes the ParA- and ParB- miniplasmid of R1 pKN1562, without increasing its copy number. The system, which we have called ParD, maps inside the 1.45-kb PstI-EcoRI fragment that is adjacent to the origin of replication of the plasmid. Two proteins whose expression is coordinated are components of the system. The sequence of the PstI-EcoRI fragment was obtained. The wild-type ParD system determines in cis a basal but detectable stability.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center