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Methods Enzymol. 1987;154:164-75.

5-Fluoroorotic acid as a selective agent in yeast molecular genetics.

Abstract

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

PMID:
3323810
[Indexed for MEDLINE]

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