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Biochem Biophys Res Commun. 1987 Dec 16;149(2):755-61.

Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes.

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Theodor-Kocher-Institut, University of Bern, Switzerland.


Human blood mononuclear cells were cultured for 24 h in the presence of LPS (100 ng per 5 x 10(6) cells), and a monocyte-derived neutrophil-activating factor (NAF) was purified to apparent homogeneity from the conditioned media. The purification consisted of ammonium sulphate precipitation, gel filtration, chromatography on phosphocellulose followed by hydroxylapatite, and reversed-phase HPLC on C4 and CN-propyl columns. Amino acid sequence analysis (32 of 50 presumed residues) shows that NAF is a novel peptide with little homology to known ones. Crude and pure NAF stimulated human neutrophils to release granule enzymes and to produce superoxide and H2O2.

[Indexed for MEDLINE]

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