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Nucleic Acids Res. 1987 Dec 23;15(24):10133-44.

Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift.

Author information

1
Biochemisch Laboratorium, Vrije Universiteit, Amsterdam, The Netherlands.

Abstract

Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene, we could show that the increase in mRNAs is a transcriptional event, mediated through DNA sequences upstream of the ribosomal protein (rp) genes. Further analysis revealed that sequence elements are involved that many rp-genes have in common and that previously were identified as transcription activation sites (RPG-boxes or UASrpg). Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.

PMID:
3320961
PMCID:
PMC339935
DOI:
10.1093/nar/15.24.10133
[Indexed for MEDLINE]
Free PMC Article

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