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Diabetologia. 1987 Jun;30(6):394-6.

Haemolysis affects insulin but not C-peptide immunoassay.

Author information

1
Diabetes Research Laboratories, John Radcliffe Hospital, Oxford, UK.

Abstract

Venous blood was taken at the end of a glucose infusion test in 19 individuals and divided into four aliquots, 3 of which were variably haemolysed by repeated passage through a 23-gauge needle to simulate traumatic venepuncture. Plasma insulin (measured by both a charcoal separation and a double-antibody method), C-peptide, and haemoglobin were measured in each aliquot, and haemolysis was also assessed visibly. A significant loss of immuno-assayable plasma insulin was found in samples with only a trace of visible haemolysis, with up to 90% lost in severely haemolysed samples. Plasma C-peptide was unaffected by haemolysis. This represents an additional advantage for the use of plasma C-peptide in assessing insulin secretion.

PMID:
3315796
DOI:
10.1007/bf00292540
[Indexed for MEDLINE]

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