Send to

Choose Destination
Diabetologia. 1987 Jun;30(6):394-6.

Haemolysis affects insulin but not C-peptide immunoassay.

Author information

Diabetes Research Laboratories, John Radcliffe Hospital, Oxford, UK.


Venous blood was taken at the end of a glucose infusion test in 19 individuals and divided into four aliquots, 3 of which were variably haemolysed by repeated passage through a 23-gauge needle to simulate traumatic venepuncture. Plasma insulin (measured by both a charcoal separation and a double-antibody method), C-peptide, and haemoglobin were measured in each aliquot, and haemolysis was also assessed visibly. A significant loss of immuno-assayable plasma insulin was found in samples with only a trace of visible haemolysis, with up to 90% lost in severely haemolysed samples. Plasma C-peptide was unaffected by haemolysis. This represents an additional advantage for the use of plasma C-peptide in assessing insulin secretion.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center