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Exp Cell Res. 1987 Sep;172(1):101-9.

Regulation of c-myc transcription and protein expression during activation of normal human B cells.

Author information

1
Department of Pathology, Norwegian Radium Hospital, Montebello, Oslo.

Abstract

The close link between an actively expressed c-myc gene locus and cellular proliferation has been established in a variety of cell types. By using normal human peripheral blood B cells as a model for in vivo quiescence, we have assessed the expression pattern of the c-myc gene in terms of transcriptional activation and concurrent production of c-myc protein, following induction by antibodies directed against antigens on the cell membrane. Both the 1F5 monoclonal antibody, which reacts with the pan B cell antigen CD20, and the anti-mu could promote transcriptional activation of the c-myc gene roughly corresponding to the increased levels of cytoplasmic c-myc mRNAs. In addition, more than 80% of the B cells could be induced to express c-myc protein by either stimulus. Since treatment only with polyclonal anti-mu renders the B cells competent to proliferate in the presence of BCGF, c-myc protein expression is not per se sufficient for cell cycle progression into S phase.

PMID:
3308492
DOI:
10.1016/0014-4827(87)90097-8
[Indexed for MEDLINE]

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