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Nucleic Acids Res. 1988 Jun 10;16(11):5067-73.

The oriC unwinding by dam methylation in Escherichia coli.

Author information

1
Institute of Applied Microbiology, University of Tokyo, Japan.

Abstract

It has been shown that dam methylation is important in the regulation of initiation of DNA replication in E.coli. The question then arises as to whether dam methylation in the oriC region mediates any structural changes in DNA involved in the regulation of initiation of DNA replication. We demonstrate that the thermal melting temperature of the oriC region is lowered by adenine methylation at GATC sites. The regulation of initiation of DNA replication by dam methylation may be attributed to the ease of unwinding at GATC sites in oriC.

PMID:
3290846
PMCID:
PMC336717
DOI:
10.1093/nar/16.11.5067
[Indexed for MEDLINE]
Free PMC Article

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