Function of a yeast TATA element-binding protein in a mammalian transcription system

S Buratowski; S Hahn; P A Sharp; L Guarente

doi:10.1038/334037a0

Function of a yeast TATA element-binding protein in a mammalian transcription system

Nature. 1988 Jul 7;334(6177):37-42. doi: 10.1038/334037a0.

Authors

S Buratowski¹, S Hahn, P A Sharp, L Guarente

Affiliation

¹ Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

PMID: 3290687
DOI: 10.1038/334037a0

Abstract

Saccharomyces cerevisiae contains a protein which is functionally similar to the mammalian TATA element-binding transcription factor, TFIID. The yeast factor substitutes for TFIID in a mammalian RNA polymerase II in vitro transcription system, forms a stable preinitiation complex on the Adenovirus-2 major late promoter, and binds specifically to the TATA boxes of the viral promoter and the yeast CYC1 promoter. Interestingly, the yeast factor promotes initiation at a distance from the TATA element typical of a mammalian system.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

DNA-Binding Proteins / genetics
DNA-Binding Proteins / isolation & purification
DNA-Binding Proteins / physiology*
HeLa Cells / metabolism
Humans
Peptide Chain Initiation, Translational
Promoter Regions, Genetic
RNA Polymerase II / physiology
Regulatory Sequences, Nucleic Acid
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism
Species Specificity
Transcription Factors / isolation & purification
Transcription Factors / metabolism
Transcription Factors / physiology*
Transcription, Genetic*

Substances

DNA-Binding Proteins
Transcription Factors
RNA Polymerase II