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Cell. 1988 Jan 29;52(2):241-51.

Spindle formation and chromatin condensation in cells blocked at interphase by mutation of a negative cell cycle control gene.

Author information

1
Department of Pharmacology, Robert Wood Johnson Medical School, UMDNJ, Piscataway 08854.

Abstract

In Aspergillus nidulans the temperature-sensitive cell cycle mutation bimE7 causes chromosome condensation and pre-anaphase spindle formation to occur at restrictive temperature. By constructing double mutants between bimE7 and S phase or G2 phase mutants and blocking DNA replication with hydroxyurea, we demonstrate that bimE7 can cause chromatin condensation and spindle formation in cells held in S or G2. Thus bimE7 overrides normal control systems that prevent mitosis from prematurely occurring during S or G2. We show that bimE7 is a loss of function mutation and propose that bimE normally functions to negatively control a positive mitotic inducing factor, possibly the cell cycle gene nimA.

PMID:
3277718
[Indexed for MEDLINE]

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