Exosomal miR-1298 and lncRNA-RP11-583F2.2 Expression in Hepato-cellular Carcinoma

Marwa Matboli; Mariam E Labib; Hanaa El-Tayeb Nasser; Ahmed H F El-Tawdi; Eman K Habib; Randa Ali-Labib

doi:10.2174/1389202920666191210111849

Exosomal miR-1298 and lncRNA-RP11-583F2.2 Expression in Hepato-cellular Carcinoma

Curr Genomics. 2020 Jan;21(1):46-55. doi: 10.2174/1389202920666191210111849.

Authors

Marwa Matboli¹, Mariam E Labib¹, Hanaa El-Tayeb Nasser¹, Ahmed H F El-Tawdi¹, Eman K Habib¹, Randa Ali-Labib¹

Affiliation

¹ 1Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11381, Egypt; 2General and Plastic Surgery Department, Military Medical Academy, Cairo, Egypt; 3Anatomy Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Egypt.

Abstract

Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in the sera of patients with HCC versus control.

Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsa-miRNA-1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C (CHC) infection and 18 healthy normal volunteers.

Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity and specificity than alpha-fetoprotein (AFP) in HCC diagnosis.

Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for HCC diagnosis.

Keywords: Hepatocellular carcinoma; chronic hepatitis C; diagnosis; exosome; lncRNA; miRNA.