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Nucleic Acids Res. 1988 Dec 23;16(24):11461-7.

Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.

Author information

1
Abteilung Biophysikalische Chemie, Medizinische Hochschule Hannover, FRG.

Abstract

A macrorestriction map of the 780 kbp Mycoplasma mobile (ATCC 43663) genome was constructed. Linking fragments were identified on two-dimensional pulsed-field electrophoresis gels. Either complete double restriction digests or partial and complete single digests were separated in the first and second dimension, respectively. 19 restriction sites of four enzymes could be assigned to the map. These rapid methods do not require DNA probes and are applicable to the long-range restriction mapping of all genomes that yield resolvable patterns on two-dimensional gels.

PMID:
3264905
PMCID:
PMC339058
DOI:
10.1093/nar/16.24.11461
[Indexed for MEDLINE]
Free PMC Article

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