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J Lab Clin Med. 1988 Jul;112(1):23-7.

Erythropoietin induces rapid increases in intracellular free calcium in human bone marrow cells.

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Department of Medicine, University of Minnesota, Minneapolis.


Elevation of intracellular free calcium (Ca++) is an early activation event that occurs as a result of ligand binding in several human cell systems. In this report, erythropoietin, the major hormone governing erythroid differentiation, was found to elicit an increase in Ca++ in human bone marrow mononuclear cells. Two chelators of intracellular calcium, quin 2 and the more specific and sensitive analogue, fura-2, were used to characterize the response evoked by both recombinant and native hormone. Erythropoietin caused a rapid, dose-dependent rise (within seconds) in Ca++ in bone marrow mononuclear cells, which could be prevented by preincubation of hormone with a rabbit erythropoietin antiserum. The erythropoietin response did not occur in purified populations of T- or B-lymphocytes. These studies suggested that increased Ca++ on erythropoietin binding may be an early transmembrane signal in hormone action.

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