Diverse Secondary Metabolites from a Lichen-Derived Amycolatopsis Strain

Curr Microbiol. 2020 Sep;77(9):2104-2110. doi: 10.1007/s00284-020-02049-5. Epub 2020 May 28.

Abstract

In this study, the secondary metabolites of a lichen-derived actinomycete strain Amycolatopsis sp. YIM 130687 were investigated intensively by using three different media (4#, 302#, and 312#) for fermentation. A total of 21 compounds were isolated from the fermented extraction of the strain. The structures of all compounds were identified by the examination of HRESIMS and NMR spectra. Compounds 1-3, 5, 6, 21 were only found in the cultivation on 302# medium, while compounds 4, 9-11 were only obtained when the strain was cultured on 312# medium. On the other hand, compounds 7, 8, and 20 were only isolated from the fermentation product on 4# medium. The antimicrobial activity test showed that compound 9 had significant inhibitory effects on bacterial pathogens of Staphylococcus aureus and MRSA with the MICs of 2 μg/ml and fungal pathogens of Botrytis cinerea and Fusarium graminearum with the MICs of 1 μg/ml.

MeSH terms

  • Amycolatopsis
  • Botrytis
  • Fusarium
  • Lichens*
  • Microbial Sensitivity Tests

Supplementary concepts

  • Botrytis cinerea
  • Fusarium graminearum