Pre-protective effect of polysaccharides purified from Hericium erinaceus against ethanol-induced gastric mucosal injury in rats

Int J Biol Macromol. 2020 Sep 15:159:948-956. doi: 10.1016/j.ijbiomac.2020.05.163. Epub 2020 May 22.

Abstract

The β-glucan H6PC20 (Mw: 2390 kDa) and α-heteropolysaccharide HPB-3 (Mw: 15 kDa) were purified from the fruiting body of Hericium erinaceus according to the previous methods. Their gastroprotective activities and corresponding structure-activity relationship were studied in the ethanol-induced gastric ulcer model of rats. After intragastric administrated with H6PC20 and HPB-3 for 14 days, macroscopic and histological evaluation of gastric mucosa was improved significantly. The defense and repair factors (EGF, bFGF and PGE2) were increased, meanwhile, the inflammatory cytokines (IL-1β and TNF-α) and MDA were reduced. These results indicated that H6PC20 and HPB-3 presented gastroprotective activities with the mechanism of activating repair and defense system, decreasing the inflammatory response and alleviating the oxidative injury. Furthermore, the structure-activity relationship showed that the macromolecular β-glucan was better for repair and defense system, while the low weight molecular α-heteropolysaccharide focused on the anti-inflammatory effect. The polysaccharides purified from H. erinaceus can be developed as a potential gastroprotective ingredient for applications in pharmaceutical field.

Keywords: Gastric ulcer; Gastroprotective activity; Hericium erinaceus; Purified polysaccharide.

MeSH terms

  • Animals
  • Biomarkers
  • Biopsy
  • Chemical Phenomena
  • Cytokines / metabolism
  • Disease Models, Animal
  • Epidermal Growth Factor / metabolism
  • Ethanol / adverse effects
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / isolation & purification
  • Fungal Polysaccharides / pharmacology*
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / pathology
  • Hericium / chemistry*
  • Immunohistochemistry
  • Male
  • Protective Agents / chemistry*
  • Protective Agents / isolation & purification
  • Protective Agents / pharmacology*
  • Rats
  • Stomach Ulcer / drug therapy
  • Stomach Ulcer / etiology
  • Stomach Ulcer / metabolism
  • Stomach Ulcer / prevention & control*

Substances

  • Biomarkers
  • Cytokines
  • Fungal Polysaccharides
  • Protective Agents
  • Ethanol
  • Epidermal Growth Factor