The emm-Cluster Typing System

Pierre R Smeesters; Anne Botteaux

doi:10.1007/978-1-0716-0467-0_3

The emm-Cluster Typing System

Methods Mol Biol. 2020:2136:25-31. doi: 10.1007/978-1-0716-0467-0_3.

Authors

Pierre R Smeesters^{1

2

3

4}, Anne Botteaux⁵

Affiliations

¹ Academic Children Hospital Queen Fabiola, Université libre de Bruxelles, Brussels, Belgium. psmeeste@ulb.ac.be.
² Molecular Bacteriology Laboratory, Université libre de Bruxelles, Brussels, Belgium. psmeeste@ulb.ac.be.
³ Tropical Diseases Research Group, Murdoch Children's Research Institute, Melbourne, Australia. psmeeste@ulb.ac.be.
⁴ Centre for International Child Health, University of Melbourne, Melbourne, Australia. psmeeste@ulb.ac.be.
⁵ Molecular Bacteriology Laboratory, Université libre de Bruxelles, Brussels, Belgium.

PMID: 32430811
DOI: 10.1007/978-1-0716-0467-0_3

Abstract

emm-cluster typing system allows to classify most Streptococcus pyogenes variants into 48 different emm clusters. The system correlates nicely with the host serum binding capacities of the M proteins and has been used in epidemiological surveys, strain selection, and vaccine development. Here we describe the allocation of the emm cluster based on the emm-typing defining region.

Keywords: M protein; PCR; Streptococcus pyogenes; emm-cluster typing; emm-typing.

MeSH terms

Antigens, Bacterial / analysis
Antigens, Bacterial / genetics*
Antigens, Bacterial / isolation & purification
Bacterial Outer Membrane Proteins / analysis
Bacterial Outer Membrane Proteins / genetics*
Bacterial Outer Membrane Proteins / isolation & purification
Carrier Proteins / analysis
Carrier Proteins / genetics*
Carrier Proteins / isolation & purification
Genotype
Humans
Molecular Epidemiology
Streptococcal Infections / diagnosis
Streptococcal Infections / genetics
Streptococcus pyogenes / genetics*
Streptococcus pyogenes / metabolism*
Superantigens / genetics

Substances

Antigens, Bacterial
Bacterial Outer Membrane Proteins
Carrier Proteins
Superantigens
streptococcal M protein